Figure 9.

The secreted N-terminal fragment of NijA signals the loss of cell adhesion in an MMP-independent manner. (A) Wild-type S2 cells were transiently transfected with the NijA constructs shown, either with or without the MT-GAL4 and UAS-Timp plasmids that confer Timp inducibility. Fraction of adherent cells is shown for each of the genotypes. (Column 1) Adherent fractions were normalized to the number of adherent cells in uninduced cells transformed with wild-type NijA. Error bars indicate SEM. Data from three experiments are shown in columns 2 and 3; six experiments are shown in columns 4 and 5. (B) Wild-type S2 cells were treated with dsRNA against NijA to knock down the endogenous protein levels or mock treated with no RNA. Column 2 shows the fraction of adherent cells after dsRNA treatment, normalized to the number of adherent cells in column 1 (mock-treated). Error bars indicate SEM; data from three experiments are shown.