Figure 3.

Quantification of abscission, indole-3-acetic acid (IAA) movement, internode length, and seedling shoot and root length in second-generation R1 plants. The asterisks mark azygous plant lines. Plant tissues were exposed to air or 25 μL L⁻¹ ethylene in air at 25°C. Numbers under bars denote transformant and seedling number. ND, Not determined. A and B, Leaf (stem and petiole) and flower explants were exposed to ethylene for 216 and 96 h, respectively, and the hours for 50% abscission estimated by interpolation between 24-h data points. C and D, ³H-labeled IAA in lanolin (approximately 10⁻⁷ m) was applied to distal or proximal ends, respectively, of 3-cm stem internodes and placed in air or ethylene for 20 h, after which 0.5 cm of the untreated ends were collected and soluble radioactivity measured. E, Fifty internodes were measured on three vegetatively propagated plants for each of the R1 plant lines. F and G, Seeds were germinated in the dark for 3 d on moist filter paper and then one-half of the seedlings were transferred to ethylene and one-half remained in air for another 5 d at which time shoot or root length were measured.