Figure 7.
Effect of fatty acids, phospholipids, and sphingoid bases on LPA phosphatase activity. The purified LPA phosphatase activity was measured using 2 to 3 μg of protein under the standard assay conditions with [3H]LPA in 0.1% (w/v) Triton X-100 as substrate and in the presence of various concentrations of fatty acids (A), phospholipids (B), and sphingoid bases (C). Each point is the average of two determinations and each performed in triplicate. PI, Phosphatidylinositol; PE, phosphatidylethanolamine; and PC, phosphatidylcholine.