Table II.
Formation of MAGs, DAGs, and TAGs from [3H]LPA and [14C]PA by the soluble and the membrane fractions
| [3H]LPA
|
[14C]PA
|
|||
|---|---|---|---|---|
| Soluble | Membranes | Soluble | Membranes | |
| pmol min−1 mg−1 | ||||
| MAG | 92.05 ± 10.6 | 9.46 ± 0.7 | n.d. | 0.11 ± 0.01 |
| DAG | 3.18 ± 0.2 | 2.05 ± 0.1 | 0.66 ± 0.1 | 6.92 ± 0.6 |
| TAG | 0.73 ± 0.1 | 13.87 ± 1.8 | 0.08 ± 0.01 | 19.74 ± 1.3 |
Fresh immature peanut seed (50 g) was used for preparing the soluble (924 mg) and the membrane (384 mg) fractions. The incorporation of [3H]LPA or [14C]PA (50 μM) into MAG, DAG, and TAG were monitored for 15 min at 30°C in the presence of 20 μM palmitoyl-CoA and 35 μg of protein. Values are mean ± se of four separate experiments. n.d., Not detected.