Figure 2.

AtCPK2 associated with the membrane fraction after various treatments. Microsomal membranes were isolated from transgenic plants expressing CPK2-GUS. Membrane pellets were homogenized in resuspension buffer alone or resuspension buffer containing EDTA, NaCl, Triton X-100, or SDS and incubated at 4°C for 30 min before repelleting. The resulting supernatant and pellet were assayed for GUS activity to assess the effect of the treatment on AtCPK2 membrane binding. Data shown are the percentage of GUS activity remaining in the pellet. Results from two independent experiments are shown. Asterisks indicate values that were significantly different from the buffer control (P ≤ 0.05)