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Two-phase separation of membranes from wild-type Columbia plants showing AtCPK2 accumulation in the lower phase. Equal proportions of the upper and lower phases were separated by SDS-PAGE and assayed by immunoblotting with antibodies specific for AtCPK2, H+-ATPase (plasma membrane marker), BiP (ER marker), and PM021 (mitochondrial membrane marker). U, Upper phase; L, lower phase.
