Figure 1.
The effect of malto-oligosaccharides on the incorporation of 14C from ADP[14C]Glc into isolated Arabidopsis starch granules. A, Separation of the amylopectin and amylose of wild-type starch granules using Sepharose CL2B chromatography. Fractions were mixed with an iodine solution, and the A595 was determined. B, Starch granules were incubated with ADP[14C]Glc for 1 h in the presence (black symbols) or absence (white symbols) of 1 mm maltotriose. Starch was fractionated by Sepharose CL2B chromatography and the 14C in each fraction was determined by scintillation counting. The results of two replicate experiments are shown.