Figure 4.

Analysis of the ¹⁴C-labeled, low-M_r material in the starch isolated from wild-type and dpe1 plants. A, Wild-type (white symbols) and dpe1 (black symbols) plants were supplied with ¹⁴CO₂ for 30 min after 6 h of the photoperiod followed by 4 h in darkness. Samples were harvested and processed as described in Figure 3A. The results of two replicate experiments are shown. B, Low-M_r material from wild-type (white symbols) and dpe1 (black symbols) starch (fractions 12–24 in A) was collected, debranched with isoamylase, and fractionated using Sepharose CL4B chromatography.