Figure 5.

Lhcb1 transcript accumulation under very low- and low fluence conditions. WS wild-type, eid1-5, and spa1 seedlings were grown in darkness for 3 d after the induction of germination. Etiolated seedlings were treated with 1-min red-light pulses of variable photon fluence rates to obtain different light fluences. Plants were harvested 4 h after pulse treatment, and total RNA was isolated. Lhcb1 transcript levels were measured by hybridization of the corresponding DNA probe to blots of 5 μg of total RNA. Fluorescent signals of 26S rRNA stained with ethidium bromide are shown as a loading control.