Figure 4.

Western- and northern-blot analysis of the 20S proteasome in starved and non-starved maize root tips. A, Total protein content in T0, T24-starved, and T24 + Glc root tips. Values are means of three independent experiments. B, Protein extracts (1 root tip/track) were separated by PAGE, and the proteasome was immunodetected with anti-20S proteasome antibodies. Relative immunosignal intensities are indicated in brackets. C and E, Northern-blot analysis of two transcripts encoding for β6- and α3-subunits of the maize 20S proteasome (10 μg total RNA/track). D and F, rRNA were used as a loading control. Values are representative of four independent experiments.