Figure 5.

Immunodetection of carbonyl residues in 20S proteasomes from starved and non-starved maize root tips. Purified 20S (10 μg) proteasomes from T0, T24-starved, and T24 + Glc-fed root tips were incubated with (A) or without (B) DNPH and separated by 12.5% (w/v) SDS-PAGE. Carbonyl residues were then immunodected with anti-DNP antibodies as described in “Materials and Methods.” A, Relative immunosignal intensities are indicated. C, Coomassie Brillant Blue coloration of aliquot fractions of DNPH-treated proteasomes after SDS-PAGE (2 μg/track) was used as loading controls.