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. 2002 Mar;128(3):1149. doi: 10.1104/pp.010612

Table II.

Hydrolysis of different substrates by purified 20S proteasome

Substrate Final Concentration Specific Activity
μm nmol mg−1 h−1
Suc-LLVY-AMC 100 135 ± 3.5
N-Carbobenzoxy (Cbz)-A-A-P-AMC 100 54 ± 5
Cbz-G-G-R-β-napthylamide (βNA) 170 224 ± 12
Cbz-Leu-Leu-Glu(LLE)-βNA 170 59 ± 2
L-Ala-βNA 100 n.d.
L-Leu-βNA 100 n.d.
L-Arg-βNA 100 n.d.
L-Phe-βNA 100 n.d.
L-Pro-βNA 100 n.d.
cpm μg−1 μg casein mg−1 h−1
Casein-125I 2.104 359 ± 25

Assays were performed as described in “Materials and Methods.” n.d., Not detected.