Figure 5.

Lipotoxicity in Dgat1^−/− cells. (A) Wild-type (Aa–Ac) and Dgat1^−/− (Ad–Af) mouse embryonic fibroblasts were incubated with media alone (Aa and Ad) or media supplemented with 1 mM palmitate (Ab and Ae) or 1 mM oleate (Ac and Af) for 12 h. Triglyceride accumulation was detected by Nile red staining. Bar = 20 μm. (B) Wild-type and Dgat1^−/− fibroblasts were supplemented with 1 mM palmitate or 1 mM oleate for 46 h. Cell death was assessed by PI staining and flow cytometry. For each sample, fluorescence of 10⁴ cells was assessed, and the percentage of cells with PI fluorescence was determined. The bar graph displays the median percentage of PI-positive cells from three independent measurements ± SE.