Table 1.
Fatty acid species in triglyceride
| Cells | FA supplement | FA, nmol/mg protein
|
||||
|---|---|---|---|---|---|---|
| 16:0 | 16:1 | 18:0 | 18:1 | 18:2 | ||
| CHO | None | 2.59 ± 0.04 | 0.33 ± 0.02 | 0.69 ± 0.03 | 1.59 ± 0.23 | 0.26 ± 0.08 |
| CHO | Palm | 19.56 ± 0.92 | 0.26 ± 0.04 | 1.02 ± 0.14 | 1.31 ± 0.14 | 0.17 ± 0.05 |
| CHO | Palm/oleate | 84.39 ± 0.36 | 3.74 ± 0.79 | 4.13 ± 0.74 | 20.77 ± 1.64 | 0.43 ± 0.18 |
| 25RA | None | 7.72 ± 1.34 | 0.81 ± 0.00 | 1.53 ± 0.11 | 6.87 ± 0.69 | 0.58 ± 0.16 |
| 25RA | Palm | 39.79 ± 4.44 | 1.94 ± 0.06 | 2.14 ± 0.10 | 4.74 ± 0.83 | 0.29 ± 0.04 |
| SCD | None | 1.88 ± 0.37 | 0.41 ± 0.02 | 0.30 ± 0.05 | 2.40 ± 0.29 | 0.20 ± 0.30 |
| SCD | Palm | 73.31 ± 9.50 | 13.10 ± 2.33 | 2.35 ± 0.41 | 22.75 ± 3.17 | 1.05 ± 0.24 |
CHO, 25RA, and SCD-overexpressing CHO cells (SCD) were incubated in media supplemented where indicated with 500 μM palmitate (palm) or 500 μM palmitate plus 200 μM oleate (palm/oleate) for 6 h. The amount of palmitate (16:0), palmitoleate (16:1), stearate (18:0), oleate (18:1), and linoleate (18:2) present in triglyceride pool is reported and represents the mean of three independent experiments ± SE.