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Expression of a yitJ-lacZ transcriptional fusion. Fusions were introduced into strains 168 (wild type; squares) and SA29 (metK overexpression strain; circles). Cells were grown in defined medium containing methionine, collected by centrifugation, and resuspended in the same medium (filled symbols) or in medium without methionine (open symbols). Samples were taken at 1-h intervals and assayed for β-galactosidase activity, expressed in Miller units.
