Figure 4.

Methylation modulates GR-mediated transcriptional activity in FS cells. (A) AF and FS cells were grown in the absence (−) or presence (+) of 5 μM 5-Aza-dC for 96 h, and the expression from the TAT3-Luc reporter was determined in the absence or presence of 100 nM dexamethasone. Luciferase activity was normalized against a cotransfected β-galactosidase reporter. The values presented are the average of at least three independent experiments, which were done in triplicate. (B) Detection of p16 mRNA by RT-PCR analysis in AF cells in the absence of 5-Aza-dC treatment, but not in FS cells. Treatment of FS cells with 5 μM 5-Aza-dC during an increasing amount of time (h) results in the reexpression of p16 mRNA.