Table 2.

EMSA for binding of the WT and Y54M vnd/NK-2 homeodomain to a set of 18-bp DNA oligomers

No.	Sequence	Kd, nM*		Kd, relative
		WT	Y54M	WT	Y54M
1	TGTGTCAAGTGGCTGTAG	13	135	1.0	10.3
3	--------T—--------	136	160	10.4	12.2
4	---------G--------	138	130	10.6	10.0
6	--------TG--------	211	122	16.1	9.3
A18	CTCTAATGGCTTTTTCTC	313	260	23.9	19.9
5	---C--------------	284	301	21.7	22.9

The core sequence is bold. The underlined area indicates DNA bases whose complements on the (−) strand are close to Y54 or M54, respectively, of the WT or mutant vnd/NK-2 homeodomain. Hyphens indicate identity with the sequence above. 

^*The measured affinities are apparent K_d values only.