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. 2003 Mar 10;100(6):3161–3166. doi: 10.1073/pnas.0530189100

Figure 2.

Figure 2

(A) Diagram showing the principle of cyclization with the fluorescence approach. (B) The emission spectra scanned every minute for ≈5 nM NPS156L16D in 50 units/ml T4 DNA ligase. The test sequence in this construct is three repeats of the 10-bp NPS, i.e., TATAAACGCC, that was recently investigated (14). Background is subtracted from the spectra. (Inset) The emission change with time at the fluorescein peak. The parameter settings for the spectrometer are: 485 nm excitation, 10 nm excitation and emission slits, 240 nm/min scan rate, and 800 V photomultiplier tube voltage.