Table 4.

Chromosomal clustering analysis of genes whose expression was altered specifically during senescence (M1) in two individual HMEC lines (48R and 184)

d	No. of clusters		P value	No. of clustered genes		P value
	OBS	RE		OBS	RE
Up-regulation in 48R
1 kb	2	0.11	<0.001	4	0.21	<0.001
2 kb	5	0.27	<0.001	10	0.55	<0.001
5 kb	8	0.68	<0.001	16	1.36	<0.001
10 kb	12	1.15	<0.001	24	2.31	<0.001
20 kb	14	1.90	<0.001	29	3.81	<0.001
50 kb	16	3.75	<0.001	34	7.53	<0.001
100 kb	21	6.64	<0.001	44	13.4	<0.001
200 kb	24	11.2	<0.001	53	22.8	<0.001
500 kb	33	22.6	0.004	72	46.9	<0.001
1 Mb	45	35.6	0.016	100	75.6	0.002
2 Mb	59	52.1	0.054	144	115.7	0.001
5 Mb	76	73.0	0.192	209	179.6	<0.001
Up-regulation in 184
1 kb	2	0.05	<0.001	4	0.09	<0.001
2 kb	2	0.08	<0.001	4	0.15	<0.001
5 kb	4	0.21	<0.001	8	0.42	<0.001
10 kb	5	0.33	<0.001	10	0.66	<0.001
20 kb	6	0.60	<0.001	12	1.21	<0.001
50 kb	7	1.11	<0.001	14	2.23	<0.001
100 kb	7	1.83	<0.001	14	3.69	<0.001
200 kb	8	3.23	0.008	16	6.56	0.008
500 kb	12	6.92	0.009	24	14.1	0.016
1 Mb	18	11.5	0.006	37	23.7	0.009
2 Mb	22	18.4	0.123	46	39.1	0.162
5 Mb	29	30.4	0.610	69	68.4	0.447

The occurrence of perturbed gene expression in two or more genes located within the distance shown defined a chromosomal cluster. Parameters are as indicated for Table 2.