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2-2-3 CD4+ cells are positively selected in the K14 thymus. (A) The total number of thymocytes in a representative thymus from the indicated mice. Thymocytes were stained with CD4-phycoerythrin, CD8-FITC, and biotinylated 2-2-3 clonotypic Ab followed by streptavidin–allophycocyanin. The CD4/CD8 profiles are shown in Upper and 2-2-3 clonotypic Ab levels on CD4 SP cells are shown in Lower. (B) Splenocytes were stained as in A. Numbers indicate the percentage of cells in the gates shown.
