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. 2003 Mar 10;100(6):3445–3449. doi: 10.1073/pnas.0635898100

Figure 1.

Figure 1

Genetic disruption of GM3 ganglioside synthesis. (A) Pathway of ganglioside synthesis showing the block in GM3S−/− mice. Cer, ceramide. (B) Strategy to disrupt GM3S locus. (Top) Targeting vector. Asterisk indicates that the HindIII site was disrupted. (Middle) Locus organization. (Bottom) Targeted locus. The approved gene name for GM3S is Siat9. (C) Southern blot of tail DNA showing HindIII cleaved DNA fragments corresponding to the wild-type (6.0 kb) and targeted (10.5 kb) GM3S alleles. (D) Northern blot of brain RNA from GM3S+/+ and GM3S−/− mice by using GM3S cDNA as a probe. Ethidium bromide-stained gel of RNA before blotting showing 28S and 18S RNA.