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. 2003 Mar 10;100(6):3467–3472. doi: 10.1073/pnas.0230380100

Figure 4.

Figure 4

Hp1 tropism extends to the zymogenic region of parietal-cell deficient tox176 transgenic mice. (A and B) Gastric units from the zymogenic region of age- and gender-matched normal and tox176 mice (A and B, respectively). Ablation of parietal cells (stained magenta in A with DBA) produces an increase in proliferating isthmal GEPs (green; marked in S-phase with BrdU administered 90 min before death). GEPs produce NeuAcα2,3Galβ1,4 glycans (red with MAA). Pit cells in the upper portion of gastric units are marked blue with A. anguilla agglutinin. (C) Hp1 (red) shows tropism for MAA-positive cells (green) located in the expanded isthmal domain of tox176 gastric units. (D) Transmission electron microscopy demonstrating direct interaction between Hp1 and a granule-free GEP in a gastric unit positioned within the zymogenic region of an ex-GF tox176 mouse stomach. (E) Hematoxylin and eosin-stained section of the zymogenic region. (Inset) A cluster of Hp1 (visualized as red by staining a serial section with antibodies to Hp surface proteins). (F and G) Serial sections prepared from an ex-GF tox176 mouse colonized for 8 weeks with Hp1. A prominent lymphoid aggregate containing CD4+ T cells and CD19+ B cells (red) is apparent. Nuclei are counterstained with bis-benzimide. (Bars, 25 μm in A–C; 0.5 μm in D; 100 μm in E–G.)