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. 2006 Jul 6;25(14):3257–3263. doi: 10.1038/sj.emboj.7601220

Figure 6.

Figure 6

Mutation of the TIM-binding pocket in TRAF3 abolishes the IFNα response to Sendai virus. (A) The Y440A/Q442A TRAF3 mutant no longer binds the Cardif-TIM. The indicated constructs were subjected to GST pull-down assay as above using GST alone or the Cardif-TIM fused to GST as bait. (B) Traf3−/− MEFs were reconstituted with wild-type Traf3 or the double point mutant of Traf3, which abolishes its ability to bind the Cardif-TIM. Expression was monitored by immunoblot. (C) IFNα production by reconstituted cells 24 h following Sendai infection was monitored by ELISA.