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DCL1 is the only DCL that produces miRNAs. RNA gel blots analysis of 10 μg total RNA prepared from rosette leaves of wild-type Col and dcl1 (A), and inflorescences of wild-type Col, dcl1, dcl2 dcl3 dcl4 triple mutants and dcl1 dcl2 dcl3 dcl4 quadruple mutant (B). The blots were successively probed with DNAs complementary to miRNAs and U6, which served as a loading control.
