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Production of ta-siRNAs in plants infected by TCV or CMV. RNA gel blot analysis of 10 μg total RNA prepared from 1-month-old rosette leaves of wild-type Col mock-inoculated or inoculated with CMV or TCV. RNAs were extracted from pools of 2–10 plants, 21 days after inoculation. The blots were probed with DNAs complementary to ta-siRNAs and miRNAs, and U6, which served as a loading control.
