Skip to main content
. 2006 Jun 29;25(14):3286–3297. doi: 10.1038/sj.emboj.7601212

Figure 2.

Figure 2

The N-terminal region of Daxx binds to RASSF1C. (A) RASSF1 binds Daxx in COS7 cells. COS7 cells were cotransfected with pCMV-HA-Daxx plus pCMV-Myc-RASSF1A or pCMV-Myc-RASSF1C. Myc-RASSF1A or Myc-RASSF1C was immunoprecipitated using anti-Myc antibody and immunoblotted with anti-HA antibody. (B) Endogenous RASSF1C binds endogenous Daxx. Lysates of untreated HeLa cells were immunoprecipitated with anti-Daxx antibody and immunoblotted with anti-RASSF1C antibody. Mock IP, lysates were immunoprecipitated with control IgG. (C) Mapping of the RASSF1C-interacting domain of Daxx. COS7 cells were cotransfected with HA-Daxx full-length or the indicated HA-Daxx deletion mutants plus Myc-RASSF1C. Immunoprecipitation and immunoblotting were carried out with anti-Myc and anti-HA antibodies, respectively. Numbers indicate amino-acid positions in the full-length Daxx protein. (D) Schematic description of the Daxx deletion mutants used in (C) and their binding to RASSF1C. PAH, paired amphipathic alpha helices domain; AD, acid-rich region; NLS, nuclear localization signal.