Skip to main content
. 2006 Jun 29;25(14):3286–3297. doi: 10.1038/sj.emboj.7601212

Figure 4.

Figure 4

The degradation of Daxx promotes the release of RASSF1C from the nucleus. (A) Dissociation of RASSF1C from Daxx in response to DNA damage. COS7 cells expressing HA-Daxx and Myc-RASSF1C were treated with MMS or UV and lysates prepared at the indicated time points were immunoprecipitated and immunoblotted with anti-Myc and anti-HA antibodies, respectively. (B) Reduction of RASSF1C in the nucleus. COS7 cells expressing Myc-RASSF1C were treated with MMS or UV and lysates were prepared at the indicated time points. The nuclear fraction was isolated and immunoblotted with anti-Myc antibody. Nucleolin and β-actin served as a nuclear marker and a cytosolic marker, respectively. (C) Daxx overexpression suppresses RASSF1C release from the nucleus. COS7 cells coexpressing Myc-RASSF1C and HA-Daxx were treated with MMS or UV and the nuclear fractions of lysates were immunoblotted as in (B).