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. 2006 Jun 29;25(14):3286–3297. doi: 10.1038/sj.emboj.7601212

Figure 6.

Figure 6

Endogenous RASSF1C is translocated from the nucleus to the cytoplasmic microtubules in response to DNA damage. (A) HeLa cells were left untreated or treated with MMS and immunostained with anti-RASSF1C and anti-β-tubulin antibodies at 3.5 h post-treatment. White arrowheads indicate the accumulation of endogenous RASSF1C at the microtubules. White dots outline the nucleus. Scale bar, 5 μm. (B) Microtubule cosedimentation. HeLa cells transfected with pCMV-HA-Daxx or pCMV empty vector were left untreated or treated with MMS. The cell lysates were incubated with taxol to stabilize microtubules. The microtubule-binding fraction was isolated as described in Materials and methods and immunoblotted with anti-RASSF1C and anti-β-tubulin antibodies.