Figure 1.

Posterior probability plots and predicted transmembrane segments for a protein whose localisation of the C-terminal was missed by HMM-TM (YDGG_ECOLI). In the upper graph we can see the unconstrained prediction. In the lower part, we can see the conditional prediction, after incorporating the information concerning the experimentally verified localisation of the C-terminus. The red bars indicate the predicted transmembrane segments, and we observe that these change also, coming in agreement with the other predictors.