Figure 6.

Wounding shortens the cell cycle time of actively cycling lens epithelial cells in the germinative zone. Twenty-four hours after lens scrape wounds were created, mice received a pulse of BrdU intraperitoneally. Twenty-four hours later, these mice received another pulse of ³H-TdR, were sacrificed 1 hour later, and eyes were processed for BrdU immunohistochemistry and autoradiography. Within the germinative zone (A-C), actively cycling (TA) cells are detected as BrdU stained (red) or cells with silver grains in the nuclei. Occasionally double-labeled cells (arrow in panel A; cells in panels B,C) are detected, indicating that a TA cell has undergone two divisions with 24-hours.