Figure 2.

Generation of Transgenic Plants Expressing a Bacterial DHPS in a Homozygous LKR/SDH Knockout Background.

(A) Arabidopsis plants were transformed with the phaseolin-DHPS construct, and expression of the bacterial DHPS in seeds was tested by protein gel blot analysis using antibodies raised against the bacterial DHPS (Shaul and Galili, 1992). Lanes 1 to 7 represent independently transformed lines. WT, wild type.

(B) and (C) Confirmation of the LKR/SDH knockout × phaseolin-DHPS plant was performed by protein gel blot analysis with anti-LKR (B) and anti-bacterial DHPS (C) antibodies. Lanes 1, 2, and 3 represent proteins extracted from a transgenic plant expressing the bacterial DHPS alone, the LKR/SDH knockout × phaseolin-DHPS candidate, and a homozygous LKR/SDH knockout mutant, respectively.