Table 1.

Relative Levels (mol %) of Free Amino Acids in Mature Seeds of Different Arabidopsis Genotypes

Amino Acid	Wassilewskija	K/O	DHPS/Ht	DHPS/Hm	K/O × DHPS
Asp	7.82 ± 0.45	6.59 ± 0.35	7.74 ± 0.46	6.33 ± 0.62	1.31 ± 0.04
Glu	39.75 ± 1.15	33.74 ± 0.58	35.00 ± 1.95	34.04 ± 1.14	10.65 ± 0.32
Asn	14.77 ± 1.04	19.61 ± 0.40	16.04 ± 1.04	14.69 ± 0.34	21.10 ± 0.49
Ser	9.36 ± 0.10	9.78 ± 0.41	7.56 ± 0.55	6.16 ± 0.27	4.52 ± 0.08
Gln	1.26 ± 0.09	1.46 ± 0.13	2.69 ± 0.43	2.75 ± 0.33	4.91 ± 0.60
Gly	2.07 ± 0.11	2.03 ± 0.05	2.04 ± 0.12	1.95 ± 0.16	2.02 ± 0.13
His	0.39 ± 0.04	0.36 ± 0.01	0.38 ± 0.10	0.37 ± 0.07	0.88 ± 0.07
Arg	0.58 ± 0.10	0.73 ± 0.08	1.24 ± 0.41	0.59 ± 0.06	0.37 ± 0.06
Thr	3.51 ± 0.16	3.29 ± 0.08	2.65 ± 0.12	2.34 ± 0.20	0.73 ± 0.02
Ala	9.53 ± 0.26	10.08 ± 0.33	7.38 ± 0.63	6.67 ± 0.23	3.91 ± 0.20
Pro	2.92 ± 0.13	2.93 ± 0.16	3.73 ± 0.85	3.05 ± 0.24	3.00 ± 0.44
Tyr	0.46 ± 0.08	0.50 ± 0.03	0.36 ± 0.07	0.31 ± 0.04	0.18 ± 0.02
Val	4.99 ± 0.28	5.34 ± 0.24	4.47 ± 0.24	4.24 ± 0.13	3.08 ± 0.14
Met	0.40 ± 0.02	0.51 ± 0.03	4.44 ± 0.75	12.80 ± 2.04	15.72 ± 0.68
Ile	1.24 ± 0.07	1.32 ± 0.18	1.17 ± 0.06	1.11 ± 0.05	0.97 ± 0.05
Leu	0.61 ± 0.05	0.77 ± 0.04	0.58 ± 0.04	0.52 ± 0.05	0.51 ± 0.04
Lys	0.33 ± 0.02	1.63 ± 0.20	2.55 ± 0.44	4.48 ± 0.82	26.38 ± 0.81

The genotypes used are as follows: Wassilewskija, control nontransformed wild type; K/O, LKR/SDH knockout; DHPS/Ht and DHPS/Hm, transgenic plants expressing the phaseolin-DHPS construct in the heterozygous and homozygous states, respectively; K/O × DHPS, LKR/SDH knockout × phaseolin-DHPS. Values represent means of at least five repeats plus or minus standard errors.