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. 2003 Apr;15(4):885–898. doi: 10.1105/tpc.009845

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Copyright © 2003, American Society of Plant Biologists

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Figure 1. — ARC1 and SRK Constructs Tested in Tobacco BY-2 Cells, and the Subcellular Localization of myc-ARC1 and GST-SRK.

(A) Predicted protein motifs in ARC1. Numerals at top indicate the positions of the motifs in the amino acids sequence.

(B) Schemes of the various ARC1 and SRK fusion proteins examined in BY-2 cells.

(C) Subcellular localization of transiently expressed myc-ARC1, GST-SRK, and GST-tSRK in BY-2 cells at 20 h after biolistic bombardment. Cytosolic localization is observed for all proteins expressed individually. A differential interference contrast (DIC) image of the myc-ARC1–expressing cell is shown.

(D) Time-course study of BY-2 cells cobombarded with myc-ARC1 and GST-SRK and allowed to express proteins for 4, 8, and 20 h. myc-ARC1 is localized to the cytosol at 4 h and then relocalizes to several distinct subcellular sites by 8 h and remains there at 20 h. Cytosolic localization is observed for GST-SRK in all cells.

(E) Control cotransformation of BY-2 cells with myc-ARC1 and GST-tSRK at 20 h. Cytosolic localization is observed for both myc-ARC1 and the inactive form of GST-SRK.