Table 2.
Higher Order Complex Formation as Determined by a Yeast GAL4 Three-Hybrid Assay
| Ternary Complex | −LTA, 30°C | −LTH + 10 mM 3AT, 30°C |
|---|---|---|
| BD-pMADS1 + pRED-FBP1 + AD-FBP2 | ++ | ++ |
| BD-pMADS1 + pRED-FBP1 + AD-FBP4 | ++ | ++ |
| BD-pMADS1 + pRED-FBP1 + AD-FBP5 | + | + |
| BD-pMADS1 + pRED-FBP1 + AD-FBP9 | − | − |
| BD-pMADS1 + pRED-FBP1 + AD-pMADS12 | − | − |
| BD-pMADS1 + pRED-FBP1 + AD-FBP23 | − | − |
| BD-pMADS1 + pRED-pMADS2 + AD-FBP2 | ++ | ++ |
| BD-pMADS1 + pRED-pMADS2 + AD-FBP4 | ++ | ++ |
| BD-pMADS1 + pRED-pMADS2 + AD-FBP5 | − | − |
| BD-pMADS1 + pRED-pMADS2 + AD-FBP9 | − | − |
| BD-pMADS1 + pRED-pMADS2 + AD-pMADS12 | − | − |
| BD-pMADS1 + pRED-pMADS2 + AD-FBP23 | − | − |
The class-B proteins pMADS1, FBP1, and pMADS2 are in BD and pRED vectors, whereas the FBP2-like genes are inserted in the AD vector. ++ indicates strong interaction, + indicates weak interaction, and − indicates no growth of the yeast cells on the selective medium. A, adenine; 3AT, 3-amino- 1,2,4-triazole; H, His; L, Leu; T, Thr.