Figure 2.

Subcellular Localization of PK12 and SR1 Fusion Proteins.

Confocal optical sections are shown of viable BY-2 cells that overexpress either PK12 or SR1 proteins fused to GFP and dsRED, respectively, or cells that overexpress both PK12:GFP and RED:SR1. The panels at right show color overlays. The fluorescence images displaying either GFP or dsRED signal are shown together with the corresponding Nomarski images. Bars = 10 μm.

(A) PK12:GFP.

(B) PK12^K125R:GFP.

(C) PK12^RAQ:GFP shown in cells exhibiting nuclear ring structure.

(D) GFP.

(E) PK12:GFP and RED:SR1.

(F) PK12^K125R:GFP and RED:SR1.

(G) PK12^RAQ:GFP shown in cells displaying nuclear ring structure and RED:SR1.

(H) RED:SR1.