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. 2003 Apr;15(4):926–938. doi: 10.1105/tpc.011056

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Copyright © 2003, American Society of Plant Biologists

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Figure 3. — Expression and Activity of PK12:HA and Its Derivatives in Transgenic Plant Lines.

(A) Protein gel blot analysis. Equal amounts of total protein were fractionated by SDS-PAGE, transferred to nitrocellulose, and probed with antibodies raised against HA. Four homozygous independent lines with high (lines 9 and 10) and low (lines 1 and 11) wild-type PK12:HA ex-pression levels, and homozygous lines that highly express mutant PK12^K125R:HA or PK12^RAQ:HA, are shown.

(B) In vitro kinase assay. PK12:HA and its mutant variants were immunoprecipitated from the transgenic lines as shown in (A). The immunoprecipitate then was subjected to an in vitro kinase assay using MBP as a substrate.