Table 1.
Upregulated and Downregulated Transcripts in the PK12 LAMMER Kinase Overexpression Linea
| Upregulated Genes | Downregulated Genes |
|---|---|
| Protein destination | Energy |
| At4g24190: Hsp90-7, 2.4 ± 0.7 (5 ESTs), 2.1 ± 0.2 (2 ESTs) | At1G44575: psbS mRNA, 0.3 ± 0.1, 0.4 ± 0.1 |
| At3g09440: HSP70, 2.2 ± 0.5, 2.0 ± 0.2 (2 ESTs) | At1g29930: PSII cab protein, 0.4 ± 0.1 (12 ESTs), 0.4 ± 0.1 |
| At5g28540: BiP, 2.4 ± 0.4, 2.7 ± 0.8 | At1g31330: PSI reaction center III, 0.4 ± 0.1 (3 ESTs), 0.5 ± 0.1 |
| At5g19180: ECR1, 3.5 ± 0.9, 2.5 ± 1 | At4g12800: PSI chain XI, 0.4 ± 0.1(5 ESTs), 0.6 ± 0.03 |
| At2g26990: CSN2, 3.9 ± 2.7, 3.0 ± 1 | At3g61470: Lhca2, 0.4 ± 0.1 (12 ESTs), 0.6 ± 0.04 |
| At5g10760: cnd41, 29.2 ± 10.2, 4.9 ± 2 | At4g10340: Lhcb5, 0.4 ± 0.1 (7 ESTs), 0.5 ± 0.1 |
| Cell rescue, defense, cell death, and aging | At5g38410: ssRubisco 3b, 0.4 ± 0.1, 0.5 ± 0.1 (10 ESTs) |
| At1g74710: ICS1, 4.2 ± 3.4 (2 ESTs), 2.0 ± 0.4 | At1g32060: PRK precursor, 0.4 ± 0.2 (7 ESTs), 0.5 ± 0.2 |
| At1g72930: RPP5-like, 5.9 ± 2.3, 3.8 ± 0.4 | Metabolism |
| At1g72910: RPP5-like, 2.7 ± 0.7, 3.2 ± 0.8 | At5g46800: carnitine acyl carrier, 0.4 ± 0.1, 0.5 ± 0.02 |
| At1g31580: CxC750, 2.5 ± 0.6, 2.4 ± 0.6 (2 ESTs) | At3g61580: sphingolipid desaturase, 0.3 ± 0.1, 0.5 ± 0.1 |
| At1g02920: GST, 6.6 ± 2.6, 2.2 ± 0.5 | At5g49360: glucosyltransferase, 0.3 ± 0.1, 0.6 ± 0.03 |
| At1g02930: GST, 2.9 ± 1.2 (7 ESTs), 2.0 ± 0.3 | At3g47340: ASN1, 0.4 ± 0.1 (2 ESTs), 0.6 ± 0.03 |
| At1g07600: metallothionein, 4.9 ± 1.6 (3 ESTs), 2 ± 0.5 | At5g49360: xylosidase, 0.4 ± 0.1 (8 ESTs), 0.4 ± 0.1 (2 ESTs) |
| At1g21310: extensin 5 3.8 ± 1.0, 5.7 ± 3.5 | Cell rescue, defense, cell death, and aging |
| Cellular communication/signal transduction | At4g35770: Sen1, 0.3 ± 0.2 (4 ESTs), 0.3 ± 0.1 (2 ESTs) |
| At1g34750: phosphatase type 2C, 1.9 ± 0.15, 1.9 ± 0.3 | Cellular communication/signal transduction |
| Metabolism | At5g21170: AKIN β1, 0.4 ± 0.1, 0.3 ± 0.1 |
| At3g51240: flavanone-3-hydroxylase, 3.0 ± 0.9 (2 ESTs), 1.7 ± 0.3 | Cellular biogenesis |
| At2g45300: EPSP, 10.0 ± 9.9 (3 ESTs), 2.4 ± 0.4 | At5g19770: tubulin α-5 chain, 0.4 ± 0.1, 0.5 ± 0.1 |
| Transcription | |
| At1g36060: RAP2.4, 0.5 ± 0.05, 0.3 ± 0.1 |
Plants analyzed were 10- and 21-day-old plants of PK12:HA line 10. Transcripts were divided into functional categories using definitions provided by MIPS (http://mips.gsf.de/proj/thal/db/tables/tables_func_frame.html). Transcripts (12) without homology with known proteins were not assigned to categories. Transcripts with a ratio of >1.6-fold change in at least three of the four repetitions in both 10- and 21-day-old plants are tabulated, and their average ratios are shown. The numbers of different ESTs that belong to the same gene are indicated in parentheses. PSI and PSII, photosystems I and II, respectively; Rubisco, ribulose-1,5-bisphosphate carboxylase/oxygenase.