TABLE 2.

PCR primers used for lipY disruption in M. tuberculosis H37Rv

Primer pair	Sequence
Primer pairs to amplify 5′ and 3′-flanks of lip Y
5′-flank
A	5′-GCTTAAGATGCCGTAGGACCCG-3′
B	5′-GGTCTAGAGACATCACCTCCGGC-3′
3′-flank
C	5′-GGAAGCTTACTCGGTATCGCCGC-3′
D	5′-GGACTAGTGGTGCAAAGTCCGGG-3′
Primer pair from the deleted segment
Δ-F	5′-GTGCAGGCATTGACAGGCGCGGCC-3′
Δ-R	5′-CCAGGTCCCCACATCGAGCCACGG-3′
Primer pairs to amplify genomic flanks in the mutants
5′-flank
E	5′-GTGACCGGGAGATCCGAGCAGAGG-3′
H1	5′-TGAGGCGATGGTGGTGTCGATGCT-3′
3′-flank
H2	5′-GGAACTGGCGCAGTTCCTCTGGGG-3′
F	5′-CCAAGGGCTGGGGTGCACAACTCC-3′