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. Author manuscript; available in PMC: 2007 Feb 17.
Published in final edited form as: J Biol Chem. 2005 Dec 14;281(7):3899–3908. doi: 10.1074/jbc.M511761200

FIGURE 5. Determination of optimal stoichiometry for reconstitution of acyl-CoA carboxylase activity.

FIGURE 5

Purified subunits were mixed in the indicated ratios prior to concentration. The molar ratios of subunits are indicated as A:B:C where A represents the ratio of the α3-subunit, B represents the ratio of the β5-subunit, and C represents the ratio of the ε-subunit. Acyl-CoA carboxylase activity with acetyl-CoA (white bars) and propionyl-CoA (black bars) substrates were measured after concentration. Results presented are the average of at least three independent experiments with a standard error.