Acetyl-CoA (A), propionyl-CoA (B), bicarbonate (C), ATP (D), and MgCl2 (E) dependence of the carboxylation was measured with reconstituted α3-β5 and α3-β5-ε. ⋄ and ♦, ACC activity of α3-β5 (⋄) and α3-β5-ε (♦). ▾ and ▴, PCC activity of α3-β5 (▾) and α3-β5-ε (▴). Results presented are the average of at least three independent experiments with a standard error.