TABLE 1.
Primers used for this study
| Primer name | Sequencea(5′→3′ direction) | Comments |
|---|---|---|
| AccA3F | GGCATATGGCTAGTCACGCCGGCT | Upstream of accA3, NdeI site added |
| AccA3R | GGAGATCTTTACTTGATCTCGGCG | Downstream of accA3, BglII site added |
| AccD4F | GGCATATGACCGTCACCGAGCCG | Upstream of accD4, NdeI site added |
| AccD4R | GGCTCGAGTCAGGCCGTGCTTGC | Downstream of accD4, XhoI site added |
| AccD5F | GGCATATGACAAGCGTTACCGAC | Upstream of accD5, NdeI site added |
| AccD5R | GGCTCGAGTCACAGGGGCACGTT | Downstream of accD5, XhoI site added |
| 1ENF | CATATGGGAACGTGCCCCTGTG | Upstream 1M of Rv3281, NdeI site added |
| 1ECR | GGATCCTCGGCGCATGTGCGTC | Downstream of Rv3281, BamHI site added |
| 102EF | GGCATATGACCGAGAAGCCGCTGC | Positions 102V of Rv3281, NdeI site added |
| 102ER | GCTCGAGTCATCGGCGCATGTGCGTC | Downstream of Rv3281, XhoI site added |
| D5–227F | CTTCGTGTACCGCCAGCAGCTGG | Internal primer of accD5, RT-PCR |
| E-139R | CTGCCGGATTGTTCGTCTCGTTC | Internal primer of Rv3281, RT-PCR |
a
Endonuclease restriction sites added to sequences are underlined.