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. 2002 Apr 15;16(8):994–1006. doi: 10.1101/gad.965102

Figure 1.

Figure 1

Biochemical purification of CKII, a FRQ phosphorylating kinase from Neurospora. (A) A diagram of the purification scheme for CKII. (B) Aliquots of the indicated final Mono Q column fractions were used for the in vitro kinase assay. B and F are the protein extracts loaded and the flow-through of the Mono Q column, respectively. The NaCl gradient is indicated at top. (C) Aliquots of the indicated Mono Q column fractions were subjected to 4%–15% SDS-PAGE, and the gel was subsequently silver stained. The three arrows denote the protein bands identified as containing the indicated CKII subunits. The two protein bands copurified with CKII were denoted by asterisks.