Figure 5.

Northern blot analyses showing that in the cka^RIP strain, the circadian oscillations of the levels of frq and clock-controlled genes were abolished, and the level of frq was high in constant darkness. (A) Cultures were harvested at the indicated time in constant darkness. The representative results of three independent Northern blot analyses of frq and ccg-2 mRNA in both wild-type and the cka^RIP strain were shown. (B–D) Densitometric analyses of the mRNA levels of frq, ccg-2, and ccg-1 under circadian cycles in the wild-type and the cka^RIP strains. In B, Northern blot results from three independent experiments described in A were used. Error bars, SD. (○) Wild type; (●) cka^RIP. (E) Northern blot analysis comparing the levels of frq in constant darkness in wild-type, cka^RIP, and frq9 strains. (F) Northern blot analysis showing that the light induction of frq and al-3 in the wild-type and cka^RIP strains. The cultures were harvested after 15 min of light treatment at DD24.