. 2003 Jan;69(1):18–23. doi: 10.1128/AEM.69.1.18-23.2003

TABLE 1.

Nucleotide sequences of agr type-specific oligonucleotide primers used in this study, and anticipated sizes of PCR products

Gene	Primer	Oligonucleotide sequence (5′-3′)^a	Size of amplified product (bp)^a	Multiplex PCR set
agr_Sa	agr1-4_Sa-1	`ATGCACATGG TGCACATGC`		1
agr-1_Sa	agr1_Sa-2	`GTCACAAGTA CTATAAGCTG CGAT`	439	1
agr-2_Sa	agr2_Sa-2	`TATTACTAAT TGAAAAGTGC CATAGC`	572	1
agr-3_Sa	agr3_Sa-2	`GTAATGTAAT AGCTTGTATA ATAATACCCA G`	321	1
agr-4_Sa	agr4_Sa-2	`CGATAATGCC GTAATACCCG`	657	1
agr-1_Se	agr1_Se-1	`GGCATTAGTC GGATTAATTA TTACG`	438	2
	agr1_Se-2	`TGTAGGCCTG CAAACGG`		2
agr-2_Se	agr2_Se-1	`TTTACCATTT GCAGCTATAC AAGTG`	575	2
	agr2_Se-2	`ATAACAATAA TATAACCAAA CTCAAAAGTA CAG`		2
agr-3_Se	agr3_Se-1	`GAAAGAGTGT ATTCAATGGA TGAGC`	338	2
	agr3_Se-2	`TAAATATTAT GTATTATATC TTCAGTATAT AAAGAGATGA`		2

Nucleotide sequences and anticipated sizes of PCR products were derived from the published sequences of alleles agr-1_Sa to agr-4_Sa and agr-1_Se to agr-3_Se (GenBank accession numbers X52543, AF001782, AF001783, AF288215, Z49220, AF346724, and AF346725, respectively).