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. 2003 Jan;69(1):707–710. doi: 10.1128/AEM.69.1.707-710.2003

TABLE 1.

Purification of GST from S. griseus

Step Protein (mg) GST activity (U) Sp act (U/mg of protein)a Yield (%) Purification (n-fold)
100,000 × g supernatant 250 1.01 0.004 100
DEAE-Sephadex 30 0.96 0.032 95.0 8
GSH-Sepharose 4B 0.15 0.48 3.2 47.5 800
a

Units of enzyme activity (U) are expressed as μmoles per minute.