FIG. 5.

DGGE analysis of the effect of PQ on colonization of a model particle system. A region of the 16S rRNA gene was targeted for PCR amplification with the 341F primer with a 40-bp GC clamp and the 534R primer. Samples were electrophoresed on a 30 to 55% denaturant DGGE gel and then stained with SYBR Green I. The gel was documented with an UVP Epi-chemi Darkroom charge-coupled device camera system. P, pelagic filtered free-living bacteria; E, ethanol control ZoBell agarose particles and replicates of ZoBell agarose particles treated with PQ. Both types of preparations were examined in triplicate, and a single representative sample was loaded on the gel due to space limitations. The open arrowheads indicate ribotypes that were lost at higher concentrations of PQ, while the solid arrowheads indicate new ribotypes that appeared.