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. 2003 Jan;69(1):533–541. doi: 10.1128/AEM.69.1.533-541.2003

FIG. 2.

FIG. 2.

Ethidium bromide-stained DGGE gel (negative image) of PCR products amplified by using the Legionella-specific LEG-448 GC clamp and LEG-858 primers for the 16S rRNA genes. Samples were taken from experimental and commercial SSF units. Lanes 2 to 8 show the changes within the Legionella populations in the sand column and water samples in the experimental SSF unit (SSF-1). Lanes 9 to 18 show comparative DGGE banding patterns obtained from the top layer sand column samples from the experimental system (SSF-1) and six commercial farms (SSF-2 to SSF-18). Lanes 1 and 9, L. pneumophila type strain; lane 2, reservoir; lane 3, storage tank; lane 4, top layer; lane 5, middle layer; lane 6, bottom layer; lane 7, headwater; lane 8, outflow water; lane 10, SSF-1; lane 11, SSF-2; lane 12, SSF-3; lane 13, SSF-4; lane 14, SSF-5; lanes 15 and 16, SSF-6 samples a and b; and lanes 17 and 18, SSF-7 samples a and b. Sample a was a sand sample taken from an SSF when it was not in use (winter period), and sample b was a sand sample taken from an SSF when it was in use. The arrowhead indicates the DGGE band from L. pneumophila.