TABLE 2.
Sequence similarity of the PCR products amplified by using the Legionella-specific LEG-448 and LEG-858 primers for the 16S rRNA genes of 27 clones from reservoir, storage tank, headwater, sand column (top, middle, and bottom layers), and outflow water samples from the experimental SSF unit
| SSF clonea | Most closely related Legionella speciesb | % Similarity | Sero- group(s)c |
|---|---|---|---|
| ofw-23 | L. birminghamensis | 96.0 | 1 |
| ofw-24 | L. birminghamensis | 95.8 | 1 |
| ofw-15 | L. erythra | 95.4 | 1 and 2 |
| ofw-35 | L. erythra, L. rubrilucens, L. quinlivanii | 93.7 | 1 and 2 |
| ofw-37 | L. lytica | 96.7 | 1 |
| Middle layer | L. maceachernii | 97.9 | 1 |
| ofw-5 | L. maceachernii | 97.9 | 1 |
| ofw-14 | L. maceachernii | 97.4 | 1 |
| ofw-31 | L. maceachernii | 97.9 | 1 |
| ofw-33 | L. maceachernii | 97.9 | 1 |
| ofw-7 | L. macdadei | 95.1 | 1 |
| Top layer | L. parisiensis | 97.4 | 1 |
| Bottom layer | L. parisiensis | 97.4 | 1 |
| Storage tank | L. parisiensis | 96.0 | 1 |
| Reservoir | L. parisiensis | 97.4 | 1 |
| Headwater | L. parisiensis | 97.2 | 1 |
| ofw-20 | L. parisiensis | 97.4 | 1 |
| ofw-21 | L. parisiensis | 97.4 | 1 |
| ofw-30 | L. parisiensis | 97.2 | 1 |
| ofw-36 | L. parisiensis | 97.4 | 1 |
| ofw-39 | L. parisiensis | 97.4 | 1 |
| ofw-17 | L. pneumophila | 96.5 | 1 |
| ofw-22 | L. pneumophila | 96.0 | 1 |
| ofw-13 | L. quinlivanii | 99.3 | 1 |
| ofw-27 | L. quinlivanii | 92.5 | 1 |
| ofw-28 | Legionella-like amoebal pathogen 1 | 99.0 | |
| ofw-34 | Legionella-like amoebal pathogen 1 | 99.3 |
ofw, outflow water.
Sequences were obtained from the Ribosomal Database Project II (34) and the EMBL DNA database (52). L. birminghamensis, L. macdadei, and L. pneumophila were isolated from hospital patients. L. erythra, L. rubrilucens, L. quinlivanii, and L. maceachernii were isolated from environmental samples. L. lytica and Legionella-like amoebal pathogen 1 were isolated from free-living amoebae. L. parisiensis was isolated from environmental samples and hospital patients.
Serogroups associated with diseases.