Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Selected primer sets for selectivity tests
| Target | Primer | Sequence | Size of PCR product (bp) | Annealing temp (°C) | Reference |
|---|---|---|---|---|---|
| oriC | P1 | TTATTAGGATCGCGCCAGGC | 163 | 55 | 25 |
| P2 | AAAGAATAACCGTTGTTCAC | ||||
| ompC | S18 | ACCGCTAACGCTCGCCTGTAT | 159 | 58 | 16 |
| S19 | AGAGGTGGACGGGTTGCTGCCGTT | ||||
| Random fragment | ST11 | AGCCAACCATTGCTAAATTGGCGCA | 429 | 60 | 1 |
| ST15 | GGTAGAAATTCCCAGCGGGTACTG | ||||
| invA | 139 | GTGAAATTATCGCCACGTTCGGGCAA | 284 | 64 | 21 |
| 141 | TCATCGCACCGTCAAAGGAACC |
