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Kinetic constants of substrates for BoNT protease activities
| Substrate (reference) | Enzyme | kcat (s−1) | Km (mM) | kcat/Km [(M−1 s−1) × 103] |
|---|---|---|---|---|
| SNAP 187-203 (17) | BoNT A | 4.7 ± 0.5 | 5.0 ± 0.5 | 0.94 |
| SNAP 187-203 | A-Lc | 23 ± 1 | 1.1 ± 0.1 | 21 |
| F1-A | A-Lc | 7.2 ± 0.4 | 0.096 ± 0.01 | 7.5 |
| VAMP 60-94 (22) | BoNT B | 24 ± 0.9 | 0.33 ± 0.03 | 72 |
| VAMP 60-94 | B-Lc | 38 ± 1 | 0.081 ± 0.004 | 470 |
| F1-B | B-Lc | 4.7 ± 0.2 | 0.027 ± 0.002 | 170 |
| VAMP 37-75 | BoNT F | 17 ± 1 | 1.1 ± 0.1 | 15 |
| F1-F | BoNT F | NDa | ND | ND |
ND, not determined.
